Contents
Explore a/b testing email marketing techniques. Get smart tactics and the right tools to improve results and boost ROI.
Even small changes in an email campaign, like a subject line or call to action, can dramatically impact results. That’s why A/B testing email marketing is so powerful. Also called split testing, it involves sending two versions of an email to different segments to see which performs better. By changing one element at a time, marketers gain insights into what truly resonates with their audience. In this article, you’ll learn how A/B testing works, what to test, tools you can use, and real-world examples. These tips and tactics will help you improve open rates, clicks, and overall email performance.
What Is A/B Testing in Email Marketing?
A/B testing, or split testing, is a method used to compare two versions of the same content to see which one performs better. In email marketing, this means sending two variants of an email. Changing just one element, like the subject line or CTA, to different audience segments.
By analyzing which version gets more opens, clicks, or conversions, marketers gain valuable insights into subscriber behavior. A/B testing helps you make data-driven decisions instead of relying on guesswork, allowing for continuous improvement in your campaigns.
Want more? Explore these proven email marketing best practices.
Why A/B Testing Matters

Make Data-Driven Decisions
A/B testing eliminates guesswork. It shows you exactly what works—based on actual subscriber behavior. Whether it’s a subject line or a CTA button, you’re not hoping for results; you’re measuring them with real data.
Boost Your Metrics
Consistent testing leads to stronger performance across:
- Open rates (by testing subject lines)
- Click-through rates (by refining CTA design or placement)
- Conversions (by optimizing layout, copy, and offers)
Even small improvements in each area can produce major gains when scaled over time.
Understand Your Audience
A/B testing reveals how different segments respond to content:
- Preferences (e.g., short vs. long emails)
- Behavior patterns (e.g., time of day engagement)
- Motivators (e.g., text vs. button CTAs)
This insight enables better segmentation and personalization, making every campaign more relevant and impactful.
Pro tip: Segment your tests for more precise insights; what resonates with one audience might not work for another.
Explore how to apply these insights to generate qualified leads.
What You Can A/B Test in an Email Campaign
A/B testing works best when you focus on one variable at a time. Here are the most effective elements to test:
1. Subject Lines
Your subject line is the first (and sometimes only) thing a subscriber sees. Try testing:
- Short vs. long formats
- Questions vs. statements
- Personalization (e.g., including the subscriber’s name)
2. Preheaders
This preview text supports the subject line and can influence open rates. Test whether a direct benefit, curiosity, or urgency performs better.
3. Email Content or Layout
Try variations in:
- Body text length and tone
- Visual vs. text-heavy formats
- Order of information or sections
Even subtle layout changes can affect engagement, especially on mobile devices.
4. CTAs (Call-to-Actions)
This is where you prompt the reader to act. Test:
- Button text (“Buy Now” vs. “Get Started”)
- Button color and size
- Placement (top, middle, or bottom of the email)
5. Send Time and Day
Timing affects visibility and engagement. Test sending:
- Early morning vs. late afternoon
- Weekday vs. weekend Track patterns and adjust based on recipient behavior.
6. From Name or Sender Identity
Who the email comes from can influence trust. Try testing:
- Company name vs. individual team member
- Formal vs. casual tone in the sender name
Each of these variables can impact performance in different ways—so start simple, test consistently, and always measure the results.
Step-by-Step Guide to A/B Testing for Email Marketing
Running a successful A/B test in email marketing doesn’t require complex tools—but it does require a clear plan. Here’s how to do it effectively:

1. Define Your Goal
Before testing anything, determine what you want to improve. Is it more opens? Higher click-through rates? Increased conversions? Your goal will dictate which element to test and how you measure success.
2. Choose One Element to Test
Focus on just one variable at a time (e.g., subject line, CTA, or send time). Testing multiple elements at once makes it hard to pinpoint what caused the change in performance.
3. Create Two Variations
Design two versions of your email: Version A (the control) and Version B (the test). Keep everything else identical to ensure you’re isolating the variable you’re testing.
4. Split Your Audience
Divide your email list into two equal, randomly selected groups. Most email platforms will handle this automatically and ensure clean segmentation.
5. Analyze Results Based on Your Chosen Metric
Once your emails have been delivered and engaged with, review the results:
- For subject lines: measure open rates
- For CTAs or content: focus on click-through and conversion rates
- For timing: analyze engagement patterns
Ensure your sample size is statistically significant before drawing conclusions.
6. Apply Learnings to Future Campaigns
If one version clearly outperforms the other, use it as a benchmark for future campaigns. Over time, A/B testing helps you build a more responsive, better-optimized email strategy.
A/B Testing Email Marketing Examples
Real-world examples show just how impactful A/B testing can be. Here are a few scenarios that highlight what’s possible with simple, focused tests:
1. Subject Line Test: 27% Higher Open Rate
A B2C retailer tested two subject lines:
- A: “Your Weekend Deal Is Here”
- B: “24-Hour Flash Sale – Save Now”
Version B saw a 27% increase in open rates, thanks to urgency and an emoji that stood out in the inbox. The lesson? Emotional triggers and clear value propositions matter.
2. CTA Button Color Test: 18% More Clicks
A SaaS company tested the CTA button color in an onboarding email:
- A: Blue (brand standard)
- B: Orange (high contrast)
The orange button in Version B generated an 18% higher click-through rate. Sometimes, visual contrast wins attention, even over brand consistency.
3. Send Time Test: 2x Engagement
A newsletter campaign tested send time:
- A: Monday at 9 a.m.
- B: Wednesday at 4 p.m.
Wednesday’s afternoon send had twice the engagement. The takeaway? Optimal timing varies by audience, and testing is the only way to know for sure.
Each of these examples reflects a single, controlled variable—and a measurable improvement. Small changes, backed by data, can lead to big gains.
Best Practices for A/B Testing in Email Marketing
To get reliable, actionable results from A/B testing, you need more than good ideas—you need a disciplined approach. Follow these best practices to ensure your tests deliver real value:

1. Test One Variable at a Time
It’s tempting to test multiple changes at once, but doing so makes it impossible to isolate what caused a difference in performance. Whether it’s the subject line, CTA, or send time, focus on just one change per test.
2. Let the Test Run Long Enough to Reach Significance
Cutting a test short may lead to misleading conclusions. Allow enough time for both versions to gather sufficient opens, clicks, or conversions. Most platforms will calculate statistical significance for you, use it to make data-backed decisions.
3. Avoid Overlapping Campaigns
Running multiple A/B tests targeting the same segment can create noise. Overlap can lead to skewed data and reduce the reliability of your results. Stagger campaigns and cleanly separate your test groups.
4. Use Reliable Testing Tools and Platforms
Platforms like Mailchimp, Brevo, and Campaign Monitor offer built-in A/B testing tools with automation, tracking, and easy-to-read reporting. Using professional tools ensures accuracy and saves time.
A consistent, methodical approach to testing is what turns average marketers into optimization pros. The key is to keep testing, and keep learning.
Tools for Running Effective A/B Tests
Choosing the right platform can make A/B testing seamless, scalable, and insightful. Below are four leading tools used by marketers to run A/B tests in email campaigns—each with strengths tailored to different needs.

1. Mailchimp
Ideal for beginners and small businesses, Mailchimp offers easy-to-use A/B testing features. You can test up to three variations at once—subject lines, send times, content blocks—and the platform automatically sends the winning version to the rest of your list. Detailed reports make it easy to interpret results and improve future campaigns.
2. Brevo (formerly Sendinblue)
Brevo is a cost-effective solution with strong automation and A/B testing capabilities. It supports basic testing for subject lines and content, making it ideal for businesses that want to experiment without getting overwhelmed. It also allows you to create automation flows with testing built-in.
3. Campaign Monitor
Campaign Monitor stands out for its design flexibility and intuitive user interface. While its A/B testing is limited to subject lines and sender details, it excels at visual elements. If design and branding are central to your strategy, this platform is a solid choice.
4. Klaviyo
Built with eCommerce in mind, Klaviyo’s A/B testing features are highly advanced. You can test within flows, segment audiences by behavior, and run data-heavy campaigns. It’s perfect for businesses with rich customer data looking to personalize at scale.
| Feature | Mailchimp | Brevo | Campaign Monitor | Klaviyo |
|---|---|---|---|---|
| Ease of Use | ★★★★☆ | ★★★★☆ | ★★★★☆ | ★★★☆☆ |
| Testable Elements | Subject lines, content, send time | Subject lines, content | Subject lines, sender | Subject lines, flows, segments |
| Automation Support | Yes | Yes | Basic | Advanced |
| Best For | Small businesses | Budget-conscious users | Design-focused teams | eCommerce brands |
| Free Plan Available | Yes (limited) | Yes | No | Yes (limited) |
Common Mistakes to Avoid
A/B testing in email marketing can deliver powerful insights, if done correctly. Many marketers miss out on those benefits by falling into common traps. Here’s what to watch for:
1. Testing Too Many Variables at Once
Changing multiple elements – like the subject line and CTA – makes it impossible to know which change actually influenced results. Stick to testing one variable per experiment to ensure clean, reliable data.
2. Ignoring Statistical Significance
Ending a test too early because one version is ahead can lead to false positives. Use your platform’s statistical significance feature to determine when results are truly meaningful, not just lucky.
3. Relying on Small Sample Sizes
A/B tests need enough data to be valid. If your list is small, consider testing only a portion of it first, then sending the winning version to the rest. Too few responses can skew results and lead to incorrect conclusions.
4. Not Documenting Results or Iterations
A/B testing is a learning process. If you don’t track what you tested and what you learned, you’re likely to repeat mistakes or miss opportunities. Keep a running log of tests, outcomes, and key takeaways to guide future campaigns.
Avoiding these pitfalls ensures your tests provide clear, actionable insights that truly optimize your email strategy.
Conclusion
A/B testing in email marketing isn’t just a tactic, it’s a strategy for long-term growth. By experimenting with key elements like subject lines, CTAs, and send times, you can uncover what resonates most with your audience and steadily improve your results.
The true power of A/B testing lies in consistency. Small, regular tests lead to meaningful insights and measurable gains. Whether you’re optimizing for opens, clicks, or conversions, the data you gather helps build smarter, more effective campaigns over time.
Need help implementing a testing strategy or analyzing your results? Contact BiziBusiness to optimize your email campaigns and turn insights into impact.




